Journal: Cell Systems

Article Title: Cold and hot fibrosis define clinically distinct cardiac pathologies

doi: 10.1016/j.cels.2025.101198

Figure Lengend Snippet:

Article Snippet: The following primary antibodies were used for staining. anti-LRP1 (cell culture, 1:100, ab92544, Abcam), anti-TIMP1 (cell culture, 1:50, AF980, R&D systems), anti-aSMA (cell culture & tissue sections, 1:400, A2547, Sigma), Anti-GFP (tissue sections, 1:100, ab6673, Abcam), anti-RFP (tissue sections, 1:200, 600-401-379, Rockland), Anti-Gal3 (tissue sections, 1:100, M3/38, Biolegend), anti-cardiac troponin T (cTnT) (tissue sections, 1:20, CT3, DSHB).

Techniques: Control, Recombinant, Synthesized, Sequencing, Mutagenesis, Software, Flow Cytometry, Electron Microscopy, Staining, Gene Expression

Journal: Nature Communications

Article Title: A nanosystem targeting tissue inhibitor of metalloproteinase-1 for continuous spatiotemporal idiopathic pulmonary fibrosis therapy

doi: 10.1038/s41467-026-68398-0

Figure Lengend Snippet: a Schematic illustration of the experimental process. (Created in BioRender. Zhang, F. (2025) https://BioRender.com/24749x6 ). b IHC staining and quantification of TIMP-1 and COL1A1 in lung sections from non-IPF controls (n = 10 non-IPF patients) and IPF patients (n = 8 IPF patients). c Correlation analysis of TIMP-1 and COL1A1. d Schematic illustration of the experimental process. (Created in BioRender. Zhang, F. (2025) https://BioRender.com/chpxtxr ). e IHC staining and quantification of TIMP-1 and COL1A1 in lung sections from controls and bleomycin-induced fibrosis mouse models. f Correlation analysis of TIMP-1 and COL1A1 (n = 20 samples). Immunofluorescence (IF) staining of ROS, IHC staining of AQP5, and the quantification of positive area in lung sections from non-IPF controls (n = 5 non-IPF patients for ROS; n = 10 non-IPF patients for AQP5) and IPF patients (n = 10 IPF patients for ROS; n = 8 IPF patients for AQP5) ( g ), as well as bleomycin-induced fibrosis mouse models (n = 10 mice) ( h ). Data indicate mean ± SEM, two-tailed unpaired Student’s t test. Source data are provided as a file.

Article Snippet: Rabbit anti-human CD63/CD9/ALIX antibody, HRP-conjugated goat anti-rabbit IgG(H+L) secondary antibody, Tetramethylrhodamine (TRITC)/FITC-conjugated goat anti-rabbit secondary antibody, rabbit anti-human TIMP-1 antibody, and mouse TIMP-1 ELISA kit were purchased from Proteintech (Wuhan, China).

Techniques: Immunohistochemistry, Immunofluorescence, Staining, Two Tailed Test

Journal: Nature Communications

Article Title: A nanosystem targeting tissue inhibitor of metalloproteinase-1 for continuous spatiotemporal idiopathic pulmonary fibrosis therapy

doi: 10.1038/s41467-026-68398-0

Figure Lengend Snippet: a Illustration of the mechanism of aT for MMP/TIMP-1 rebalance. (Created in BioRender. Zhang, F. (2025) https://BioRender.com/5c51mwj ). b , c The levels of TIMP-1 and active MMP-9 in MLF supernatants. d , e Cell apoptosis and quantification of A549 cells. f Cell viability of bleomycin-challenged A549 cells after various treatments for 24, 48, and 72 h. g Images of wound healing assay and quantification of migration area. h CLSM images, 3D maps and quantification of ROS in A549 cells. Data indicate mean ± SEM, one-way ANOVA with Dunnett’s multiple comparisons test; Measurements in Fig. were taken from 3 biological replicates. Source data are provided as a Source Data file.

Article Snippet: Rabbit anti-human CD63/CD9/ALIX antibody, HRP-conjugated goat anti-rabbit IgG(H+L) secondary antibody, Tetramethylrhodamine (TRITC)/FITC-conjugated goat anti-rabbit secondary antibody, rabbit anti-human TIMP-1 antibody, and mouse TIMP-1 ELISA kit were purchased from Proteintech (Wuhan, China).

Techniques: Wound Healing Assay, Migration

Journal: Nature Communications

Article Title: A nanosystem targeting tissue inhibitor of metalloproteinase-1 for continuous spatiotemporal idiopathic pulmonary fibrosis therapy

doi: 10.1038/s41467-026-68398-0

Figure Lengend Snippet: a Illustration of animal experiment procedure. (Created in BioRender. Zhang, F. (2025) https://BioRender.com/15qup8o ). Representative images ( b ), Masson staining ( c ), and Ashcroft score ( d ) of mouse lungs after different treatments (n = 6 per group). e IF staining of α-SMA and COL1A1 in mouse lung sections (n = 6 per group). f HYP content in mouse lung tissues (n = 6 per group). The level of TIMP-1 ( g ) and active MMP-9 ( h ) in mouse BALF (n = 4 per group). i Flow cytometry analysis of the ROS levels in mouse lung tissues (n = 5 per group). j TUNEL staining of mouse lung sections (n = 6 per group). Data indicate mean ± SEM; one-way ANOVA with Dunnett’s multiple comparisons test. Source data are provided as a file.

Article Snippet: Rabbit anti-human CD63/CD9/ALIX antibody, HRP-conjugated goat anti-rabbit IgG(H+L) secondary antibody, Tetramethylrhodamine (TRITC)/FITC-conjugated goat anti-rabbit secondary antibody, rabbit anti-human TIMP-1 antibody, and mouse TIMP-1 ELISA kit were purchased from Proteintech (Wuhan, China).

Techniques: Staining, Flow Cytometry, TUNEL Assay